New Construct Approaches for Efficient Gene Silencing in Plants
نویسندگان
چکیده
منابع مشابه
New construct approaches for efficient gene silencing in plants.
An important component of conventional sense, antisense, and double-strand RNA-based gene silencing constructs is the transcriptional terminator. Here, we show that this regulatory element becomes obsolete when gene fragments are positioned between two oppositely oriented and functionally active promoters. The resulting convergent transcription triggers gene silencing that is at least as effect...
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In plants, transgenes with inverted repeats are used to induce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self...
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Homology-dependent gene silencing phenomena in plants have received considerable attention, especially when it was discovered that the presence of homologous sequences not only affected the stability of transgene expression, but that the activity of endogenous genes could be altered after insertion of homologous transgenes into the genome. Homology-mediated inactivation most likely comprises at...
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Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism. In plants infected with unmodified viruses the mechanism is specifically targeted against the viral genome. However, with virus vectors carrying inserts derived from host genes the process can be additionally targeted against the corresponding mRNAs. VIGS has been used widely in plants...
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Epigenetic regulation of gene expression is a heritable change in gene expression that cannot be explained by changes in gene sequence. It can result in the repression or activation of gene expression and is therefore referred to as gene silencing or gene activation respectively. Until the end of the 1980s, only modifications of DNA or protein that lead to transcriptional repression or activati...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2006
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.106.082271